Search results for "Optical biosensing"

showing 3 items of 3 documents

Characterization of porous alumina membranes for efficient, real-time, flow through biosensing

2015

Abstract Nanofluidic sensing devices promise high performance by overcoming issues of mass transport of analyte molecules to the sensing surface, whilst micro-porous membranes promise high sensitivity due to a large surface for their capture. Anodic alumina (AAO) filter membranes allow the flow through of samples, and could be used as a convenient and readily available fluidic platform for the targeted delivering of analytes to bioreceptors immobilized on the pore walls. The relatively small pore dimensions, compared to fluidic diffusion lengths, promise highly efficient capture of analytes from the whole sample volume, enabling relatively fast sensing response times and the use of small sa…

AnalyteMaterials sciencegenetic structuresQuantum dotsDiffusionFiltration and SeparationNanotechnologyPorous aluminaPore size distributionBiochemistryCharacterization (materials science)AnodeMembraneGeneral Materials ScienceFluidicsPhysical and Theoretical ChemistryPorosityBiosensorOptical biosensing and sensorsProtein physisorption
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Optimization of fluorescence enhancement for silicon-based microarrays

2008

An optical technique for the enhancement of fluorescence detection sensitivity on planar samples is presented. Such a technique is based on the simultaneous optimization of excitation and light collection by properly combining interference and reflectance from the sample holder. Comparative tests have been performed in microarray applications, by evaluating the proposed solution against commercial glass-based devices, using popular labeling dyes, such as Cy3 and Cy5. The proposed technique is implemented on a substrate built with standard silicon technology and is therefore well suited for integrated micro total analysis systems (microTAS) applications.

Quality ControlSiliconMaterials scienceSiliconBiomedical Engineeringchemistry.chemical_elementSubstrate (electronics)computer.software_genreSensitivity and SpecificitySettore ING-INF/01 - ElettronicaFluorescence spectroscopyBiomaterialsOpticsPlanarInterference (communication)Computer Aided DesignDetection theorySensitivity (control systems)Microscopy Confocalbusiness.industryoptical biosensingReproducibility of ResultsEquipment DesignImage EnhancementMicroarray AnalysisAtomic and Molecular Physics and OpticsElectronic Optical and Magnetic MaterialsEquipment Failure AnalysisMicroscopy FluorescencechemistryComputer-Aided DesignbusinesscomputerJournal of Biomedical Optics
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Real time optical immunosensing with flow-through porous alumina membranes

2014

Through the presentation of analytical data from bioassay experiments, measured by polarimetry, we demonstrate for the first time a real time immunoassay within a free standing macroporous alumina membrane. The 200 nm nominal pore diameter of the membrane enables flow-through, thereby providing an ideal fluidic platform for the targeted delivery of analytes to bioreceptors immobilized on the pore walls, enabling fast sensing response times and the use of small sample volumes (<100 mu L). For the immunoassay, the pore walls were first coated with the functional copolymer, copoly(DMA-NAS) using a novel coupling process, before immobilization of the allergen protein, beta-lactoglobulin, by spo…

StreptavidinAnalyteOptical biosensingMaterials scienceAnalytical chemistryPorous aluminaquantum dotsForm birefringencechemistry.chemical_compoundCopolymerPolarimetryMaterials ChemistryCopolymermedicineFluidicsAnnan elektroteknik och elektronikElectrical and Electronic EngineeringPorosityInstrumentationcopolymerOther Electrical Engineering Electronic Engineering Information Engineeringmedicine.diagnostic_testQuantum dotsMetals and AlloysCondensed Matter PhysicsNoise floorSurfaces Coatings and FilmsElectronic Optical and Magnetic MaterialsMembranechemistryImmunoassay
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